BioTek Synergy HT Multi-Mode Microplate Reader

The BioTek Synergy HT Multi-Mode Microplate Reader is a versatile laboratory analytical instrument designed for measuring absorbance, fluorescence and luminescence in microplate-based assays. Manufactured by BioTek Instruments, now part of Agilent Technologies, this instrument is widely used in pharmaceutical research, biotechnology, molecular biology, biochemistry, life sciences and academic research laboratories.

The Synergy HT combines multiple optical detection technologies in one benchtop system, allowing researchers to perform different types of laboratory assays using a single instrument. Its dual-optics design incorporates separate optical channels for absorbance and fluorescence measurements, providing flexibility for a wide range of scientific applications.

The instrument is particularly useful for laboratories performing enzyme-linked immunosorbent assays (ELISA), protein quantification, nucleic acid analysis, cell viability testing, enzyme activity measurements and fluorescence-based research. It also supports luminescence measurements, making it suitable for applications involving ATP detection, reporter gene assays and other light-emitting biochemical reactions.

One of the main capabilities of the BioTek Synergy HT is its UV-visible absorbance measurement system. It uses a xenon flash lamp and monochromator-based wavelength selection to measure absorbance over a wavelength range of 200 to 999 nm, with wavelength selection in 1 nm increments. This makes it suitable for measuring optical density, analyzing colorimetric reactions and performing quantitative laboratory assays.

The instrument also features a fluorescence detection system using a tungsten quartz-halogen light source, interference filters and a photomultiplier tube (PMT) detector. It supports both top and bottom fluorescence measurements, providing flexibility for different sample types and experimental configurations. The standard fluorescence wavelength ranges include excitation from 300 to 650 nm and emission from 300 to 700 nm. Optional configurations may provide extended wavelength capabilities.

For luminescence-based applications, the Synergy HT uses a low-noise PMT detector to measure light emitted from chemical and biological reactions. This capability is useful for ATP assays, cellular luminescence measurements, reporter gene analysis and luminescent immunoassays. The luminescence detection system can be used with compatible microplates and assay reagents.

The BioTek Synergy HT supports multiple microplate formats, including 6-, 12-, 24-, 48-, 96- and 384-well plates. This flexibility allows laboratories to perform experiments using different sample volumes and plate configurations. It is particularly beneficial for laboratories handling large numbers of samples or conducting repeated assay measurements.

Another important feature is its integrated temperature control system. The instrument incorporates BioTek's 4-Zone temperature control technology, which supports temperature regulation from approximately 4°C above ambient temperature to 50°C. Temperature control is particularly useful for kinetic assays, enzyme reactions and other experiments requiring stable thermal conditions.

The Synergy HT also includes an integrated microplate shaking mechanism. Users can select different shaking speeds, including low, medium, high and variable settings, with adjustable shaking durations. This feature helps improve sample mixing and supports experimental procedures requiring consistent reaction conditions.

The instrument supports different reading methods, including endpoint reading, kinetic measurement, spectral scanning and well-area scanning. Endpoint measurements are useful for assays requiring a single reading, while kinetic measurements allow researchers to monitor changes in absorbance, fluorescence or luminescence over time. Spectral scanning provides additional flexibility for analyzing samples across different wavelengths.

The system is computer-controlled and compatible with BioTek's Gen5 and KC4 data analysis software, depending on instrument and software versions. These software platforms allow users to configure experimental protocols, control measurement parameters, collect analytical data and perform data processing. The Synergy HT is also designed for compatibility with BioTek BioStack microplate automation systems, supporting automated laboratory workflows.

Optional configurations include time-resolved fluorescence capability and dual-reagent dispensing systems. These options can expand the instrument's applications in advanced biochemical research, pharmaceutical screening and specialized assay development. Their availability depends on the specific instrument model and installed accessories.

The BioTek Synergy HT is suitable for pharmaceutical quality control laboratories, biotechnology research facilities, university laboratories, clinical research laboratories, molecular biology facilities and industrial research and development departments.

Overall, the BioTek Synergy HT Multi-Mode Microplate Reader provides a practical combination of absorbance, fluorescence and luminescence detection technologies. Its multiple microplate formats, integrated temperature control, shaking capability and computer-based operation make it a versatile analytical solution for laboratories performing a wide variety of microplate-based research and testing procedures.

Applications of Product

1. ELISA Testing: Absorbance-based enzyme-linked immunosorbent assays.

2. Protein Quantification: Measurement of protein concentration in biological samples.

3. DNA and RNA Analysis: Nucleic acid quantification using UV absorbance.

4. Cell Viability Assays: Evaluation of cell survival and proliferation.

5. Cytotoxicity Studies: Analysis of cellular responses to compounds.

6. Enzyme Kinetics: Monitoring enzyme activity and reaction rates.

7. Fluorescence Assays: Detection of fluorescent dyes and labelled biomolecules.

8. Luminescence Assays: ATP detection and reporter gene analysis.

9. Pharmaceutical Research: Drug screening and compound evaluation.

10. Biotechnology Research: Biochemical and molecular biology investigations.

11. Immunology Research: Antibody and antigen-based assay measurements.

12. Microbial Growth Studies: Monitoring microbial growth using compatible absorbance assays.

13. Biomarker Analysis: Detection and quantification of biological markers.

2.2 Product Features

• Multi-mode microplate reading capability.

• Absorbance, fluorescence and luminescence detection.

• Dual-optics design with separate absorbance and fluorescence channels.

• Xenon flash lamp for absorbance measurements.

• Tungsten quartz-halogen lamp for fluorescence measurements.

• Photomultiplier tube (PMT) detection technology.

• UV-visible absorbance wavelength range of 200–999 nm.

• Top and bottom fluorescence measurement capability.

• Integrated 4-Zone temperature control.

• Temperature control up to 50°C.

• Built-in microplate shaking mechanism.

• Multiple programmable shaking speeds.

• Compatibility with 6- to 384-well microplates.

• Endpoint, kinetic and spectral scanning methods.

• Computer-controlled operation.

• Gen5 and KC4 software compatibility.

• Optional time-resolved fluorescence capability.

• Optional dual-reagent dispensing system.

• Compatible with BioTek BioStack automation systems.

2.3 Technical Specifications

The following specifications are based on the manufacturer's Synergy HT operator documentation and technical references.

Note: Optional detection modes, TRF capability, injector availability and software compatibility depend on the exact Synergy HT configuration. Confirm the serial number and installed options before publishing final specifications.

2.4 Product Condition

Condition: To be confirmed.

• Brand New

• Refurbished

• Used