BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader

The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader is an advanced laboratory analytical instrument designed for performing multiple types of microplate-based assays using different optical detection technologies. Manufactured by BioTek Instruments, now part of Agilent Technologies, this system combines monochromator-based and filter-based optical systems in a single compact benchtop instrument.

The Synergy H4 is designed to provide flexibility, sensitivity and reliable analytical performance for research laboratories, pharmaceutical companies, biotechnology facilities, academic institutions and drug discovery laboratories. Its hybrid optical technology enables researchers to perform a wide range of biological, biochemical and chemical measurements without requiring several separate analytical instruments.

One of the most important characteristics of the BioTek Synergy H4 is its ability to perform multiple detection modes, including fluorescence intensity, fluorescence polarization, time-resolved fluorescence, luminescence, UV-visible absorbance and AlphaScreen/AlphaLISA assays. This combination makes the instrument suitable for a broad range of research and diagnostic assay development applications.

The instrument incorporates two different optical detection technologies. The monochromator-based optical system provides flexible wavelength selection and spectral scanning capabilities. It allows researchers to select wavelengths according to their experimental requirements. The filter-based optical system uses high-performance filters and dichroic mirrors to provide enhanced sensitivity, signal detection and faster measurement performance for compatible assays.

The Synergy H4 supports UV-visible absorbance measurements, making it suitable for applications involving nucleic acid quantification, protein concentration measurement, enzyme activity analysis and colorimetric assays. Its absorbance system offers a wavelength selection range of approximately 230–999 nm, with 1 nm increments, and a dynamic range of 0–4.0 optical density.

For fluorescence-based applications, the instrument supports both top and bottom fluorescence detection. This feature is useful for researchers working with fluorescent dyes, labelled proteins, cellular assays and other fluorescence-based experimental methods. The hybrid optical configuration provides flexibility through monochromator selection while also supporting high-performance filter-based measurements.

The BioTek Synergy H4 also provides luminescence detection for applications involving light-producing chemical and biological reactions. It is suitable for ATP measurements, reporter gene assays, cell viability studies and other luminescence-based research applications. Its high-sensitivity detection system supports measurements of low-level luminescent signals.

Another important capability is time-resolved fluorescence (TRF), which is widely used in sensitive biochemical assays and fluorescence resonance energy transfer-based research. The instrument also supports fluorescence polarization measurements, which are useful for studying molecular interactions, binding reactions and drug discovery processes.

The AlphaScreen and AlphaLISA detection capabilities make the Synergy H4 particularly useful for pharmaceutical research and high-throughput screening applications. These technologies support homogeneous assay formats and are commonly used in protein interaction studies, biomarker research and drug discovery.

The system supports different microplate formats, with configurations accommodating plates ranging from single-well formats to 384-well and, with the appropriate filter-based configuration, up to 1536-well plates. This flexibility allows laboratories to perform experiments at different sample volumes and throughput levels.

The Synergy H4 supports multiple reading methods, including endpoint reading, kinetic measurement, spectral scanning and well-area scanning. Endpoint reading is suitable for measurements taken at a specific time, while kinetic reading enables researchers to monitor changes in absorbance, fluorescence or luminescence over time.

Temperature control is another useful feature for temperature-sensitive experiments. The system offers an integrated temperature control capability up to approximately 65°C, depending on configuration. Built-in plate shaking helps improve sample mixing and supports assays requiring controlled agitation.

The instrument can be operated using BioTek Gen5 software, which provides tools for instrument control, experimental protocol development, data acquisition and analysis. Researchers can configure different assay protocols, select detection methods, define measurement parameters and process experimental results through the software.

The BioTek Synergy H4 is suitable for pharmaceutical research and development, biotechnology, molecular biology, immunology, cell biology, biochemistry and analytical testing laboratories. Its combination of optical detection methods makes it a versatile solution for laboratories that perform a variety of microplate-based assays.

Overall, the BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader offers a combination of optical flexibility, multiple detection technologies and automated microplate analysis in one laboratory instrument. It is an appropriate choice for research facilities requiring versatile assay measurement capabilities for scientific investigation, pharmaceutical development and advanced biological research.

Important: Actual performance, detection modes, filters, injectors, plate compatibility and software availability depend on the installed configuration and accessories.

Applications of Product

1. Pharmaceutical Research: Drug screening, compound evaluation and pharmaceutical assay development.

2. Protein Quantification: Measurement of protein concentration using colorimetric and fluorescence-based assays.

3. DNA and RNA Analysis: Nucleic acid quantification and sample analysis.

4. ELISA Testing: Absorbance-based immunoassays and biomarker measurement.

5. Cell Biology: Cell proliferation, viability and cytotoxicity assays.

6. Enzyme Kinetics: Monitoring enzyme activity and reaction rates.

7. Fluorescence Assays: Measurement of fluorescent dyes, probes and labelled biomolecules.

8. Luminescence Assays: ATP detection and reporter gene analysis.

9. Drug Discovery: High-throughput screening and molecular interaction studies.

10. Biotechnology Research: Protein interactions and biochemical analysis.

11. Immunology: Antibody-based and immunological assay development.

12. Molecular Biology: Fluorescence-based and absorbance-based molecular analysis.

2.2 Product Features

• Hybrid monochromator and filter-based optical technology.

• Multiple detection modes in one instrument.

• UV-visible absorbance measurement.

• Top and bottom fluorescence detection.

• Fluorescence polarization (FP).

• Time-resolved fluorescence (TRF).

• Luminescence detection.

• AlphaScreen and AlphaLISA compatibility.

• Flexible wavelength selection.

• Spectral scanning capability.

• Endpoint and kinetic reading methods.

• Microplate shaking capability.

• Integrated temperature control up to approximately 65°C.

• Compatibility with multiple microplate formats.

• Gen5 software compatibility.

• Optional dual reagent injection system.

• Suitable for research and drug discovery applications.

2.3 Technical Specifications

The following specifications are compiled from available BioTek Synergy H4 product documentation and technical references. Exact specifications vary by installed configuration.

Note: Dimensions and weight are approximate reference values. Verify the instrument's exact configuration and nameplate before listing.

2.4 Product Condition

Condition: To be confirmed.

• Brand New

• Refurbished

• Used